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All experiments were being approved by the house Place of work. Animals were being housed, 6 per cage at regular temperature under a fourteen : 10 h light-weight/darkish cycle, with free of charge access to foodstuff and drinking water all the time.

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Subsequent this, unilateral L5 spinal nerve ligation was executed. 7 times later, sensory thresholds were reassessed and the event of allodynia to chilly and mechanical stimuli and hyperalgesia to a noxious thermal stimulus verified.

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